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慶應義塾大学 薬学部・大学院薬学研究科 創薬分析化学講座 Division of Analytical Chemistry for Drug Discovery Faculty of Pharmacy Graduate School of Pharmaceutical Sciences Keio University

JP | EN

研究業績

2022年

原著論文

  • Kenjiro Hanaoka, Shimpei Iwaki, Kiyoshi Yagi, Takuya Myochin, Takayuki Ikeno, Hisashi Ohno, Eita Sasaki, Toru Komatsu, Tasuku Ueno, Motokazu Uchigashima, Takayasu Mikuni, Kazuki Tainaka, Shinya Tahara, Satoshi Takeuchi, Tahei Tahara, Masanobu Uchiyama, Yasuteru Urano “A general design strategy to precisely control the emission of fluorophores via a twisted intramolecular charge transfer (TICT) process”
    J. Am. Chem. Soc., (in press).
  • Kenichi Nagase, Naoto Kojima, Mitsuaki Goto, Toshihiro Akaike, Hideko Kanazawa “Thermoresponsive block copolymer brush for temperature-modulated hepatocyte separation”
    Journal of Materials Chemistry B, (in press). DOI: 10.1039/D2TB01384C
  • Kenichi Nagase, Saki Ishii, Ayako Takeuchi, Hideko Kanazawa “Temperature-modulated antibody drug separation using thermoresponsive mixed polymer brush-modified stationary phase”
    Separation and Purification Technology, 299, 121750 (2022). DOI: 10.1016/j.seppur.2022.121750
  • Kenichi Nagase, Hikaru Takagi, Hideo Nakada, Haruki Ishikawa, Yoshiko Nagata, Tohru Aomori, Hideko Kanazawa “Chromatography columns packed with thermoresponsive-cationic-polymer-modified beads for therapeutic drug monitoring”
    Scientific Reports, 12, 12847 (2022). DOI: 10.1038/s41598-022-16928-3
  • Yugo Kuriki, Takafusa Yoshioka, Mako Kamiya, Toru Komatsu, Hiroyuki Takamaru, Kyohhei Fujita, Hirohisa Iwaki, Aika Nanjo, Yuki Akagi, Kohei Takeshita, Haruaki Hino, Rumi Hino, Ryosuke Kojima, Tasuku Ueno, Kenjiro Hanaoka, Seiichiro Abe, Yutaka Saito, Jun Nakajima, Yasuteru Urano “Development of a fluorescent probe library enabling efficient screening of tumour-imaging probes based on discovery of biomarker enzymatic activities”
    Chemical Science, 13, 4474-4481 (2022). DOI: 10.1039/D1SC06889J
  • Yuki Hoshino, Kenjiro Hanaoka, Kei Sakamoto, Masahiro Yasunaga, Takashi Kojima, Daisuke Kotani, Ayumu Nomoto, Eita Sasaki, Toru Komatsu, Tasuku Ueno, Hiroyuki Takamaru, Yutaka Saito, Yasuyuki Seto, Yasuteru Urano “Molecular design of near-infrared (NIR) fluorescent probes targeting exo-peptidase and application for detection of dipeptidyl peptidase 4 (DPP-4) activity”
    RCS Chemical Biology, 3, 859-867 (2022). DOI: 10.1039/D1CB00253H
  • Akihiro Nakada, Takuma Maruyama, Mako Kamiya, Kenjiro Hanaoka, Yasuteru Urano “Rapid visualization of deeply located tumors in vivo by intravenous administration of a γ-glutamyltranspeptidase-activated fluorescent probe”
    Bioconjugate Chemistry, 33, 523-529 (2022). DOI: 10.1021/acs.bioconjchem.2c00039
  • Kenichi Nagase, Kosuke Matsumoto, Hideko Kanazawa “Temperature-responsive mixed-mode column for the modulation of multiple interactions”
    Scientific Reports, 12(1), 4434 (2022). DOI: 10.1038/s41598-022-08475-8
  • Kenichi Nagase, Sakiko Kitazawa, Toshihiko Kogure, Sota Yamada, Kazuhiro Katayama, Hideko Kanazawa “Viral vector purification with thermoresponsive-anionic mixed polymer brush modified beads-packed column”
    Separation and Purification Technology, 286, 120445 (2022). DOI: 10.1016/j.seppur.2022.120445
  • Kenichi Nagase, So Inoue, Masakazu Inoue, Hideko Kanazawa “Two-dimensional temperature-responsive chromatography using a poly(N-isopropylacrylamide) brush-modified stationary phase for effective therapeutic drug monitoring”
    Scientific Reports, 12(1), 2653 (2022). DOI: 10.1038/s41598-022-06638-1

総説

  • 花岡 健二郎 “創薬研究のための蛍光プローブの分子設計と活用方法”
    MED CHEM NEWS, 32 (2), 87-92 (2022).
  • 佐々木 栄太, 花岡 健二郎 “非ウイルス性タンパク質シェル工学の現在と将来展望”
    JSMI Report, 15 (2), 11-16 (2022).

著書

  • 花岡 健二郎
    “蛍光プローブ”
    先端の分析法 第2版, 第3章, 1-1, pp294-298 (2022).

2021年

原著論文

  • Kenichi Nagase, Teruno Nishiyama, Masakazu Inoue, Hideko Kanazawa “Temperature responsive chromatography for therapeutic drug monitoring with an aqueous mobile phase”
    Scientific Reports, 11(1), 23508 (2021). DOI: 10.1038/s41598-021-02998-2
  • Yusuke Miyazaki, Eizo Marutani, Takamitsu Ikeda, Xiang Ni, Kenjiro Hanaoka, Ming Xian, Fumito Ichinose “A sulfonyl azide-based sulfide scavenger rescues mice from lethal hydrogen sulfide intoxication”
    Toxicological Sciences, 183, 393-403 (2021). DOI: 10.1093/toxsci/kfab088
  • Takeru Takagi, Tasuku Ueno, Keisuke Ikawa, Daisuke Asanuma, Yusuke Nomura, Shin-Nosuke Uno, Toru Komatsu, Mako Kamiya, Kenjiro Hanaoka, Chika Okimura, Yoshiaki Iwadate, Kenzo Hirose, Tetsuo Nagano, Kaoru Sugimura, Yasuteru Urano “Discovery of an F-actin-binding small molecule serving as a fluorescent probe and a scaffold for functional probes”
    Science advances, 7, eabg8585 (2021). DOI: 10.1126/sciadv.abg8585
  • Takayuki Ikeno, Kenjiro Hanaoka, Yasuteru Urano “Development of a small-molecule-based activatable photoacoustic probe”
    Methods in Enzymology, 657, 1-19 (2021). DOI: 10.1016/bs.mie.2021.06.041
  • Kouichi Yanagi, Toru Komatsu, Shusuke Ogihara, Takayoshi Okabe, Hirotatsu Kojima, Tetsuo Nagano, Tasuku Ueno, Kenjiro Hanaoka, Yasuteru Urano “Establishment of live-cell-based coupled assay system for identification of compounds to modulate metabolic activities of cells”
    Cell Reports., 36(1), 109311 (2021). DOI: 10.1016/j.celrep.2021.109311
  • Eizo Marutani, Masanobu Morita, Shuichi Hirai, Shinichi Kai, Robert M. H. Grange, Yusuke Miyazaki, Fumiaki Nagashima, Lisa Traeger, Aurora Magliocca, Tomoaki Ida, Tetsuro Matsunaga, Daniel R. Flicker, Benjamin Corman, Naohiro Mori, Yumiko Yamazaki, Annabelle Batten, Rebecca Li, Tomohiro Tanaka, Takamitsu Ikeda, Akito Nakagawa, Dmitriy N. Atochin, Hideshi Ihara, Benjamin A. Olenchock, Xinggui Shen, Motohiro Nishida, Kenjiro Hanaoka, Christopher G. Kevil, Ming Xian, Donald B. Bloch, Takaaki Akaike, Allyson G. Hindle, Hozumi Motohashi, Fumito Ichinose “Sulfide catabolism ameliorates hypoxic brain injury”
    Nature communications, 12: 3108 (2021). DOI: 10.1038/s41467-021-23363-x
  • Nagase K, Edatsune G, Nagata Y, Matsuda J, Ichikawa D, S. Yamada, Hattori Y, Kanazawa H “Thermally-modulated cell separation columns using a thermoresponsive block copolymer brush as a packing material for the purification of mesenchymal stem cells”
    Biomaterials Science, 9, 7054-7064 (2021).DOI: 10.1039/D1BM00708D
  • Soichiro Ogawa, Hidemasa Kubo, Yasutoshi Murayama, Takeshi Kubota, Masayuki Yubakami, Tatsuya Matsumoto, Takuma Ohashi, Kazuma Okamoto, Yugo Kuriki, Kenjiro Hanaoka, Yasuteru Urano, Eigo Otsuji "Matrix metalloprotease–14 is a target enzyme for detecting peritoneal metastasis in gastric cancer"
    Photodiagnosis Photodyn. Ther., 35 (2021) 102420
  • Sebastien Jenni, Flavien Ponsot, Pierre Baroux, Lucile Collard, Takayuki Ikeno, Kenjiro Hanaoka, Valentin Quesneau, Kevin Renault and Anthony Romieu “Design, synthesis and evaluation of enzyme-responsive fluorogenic probes based on pyridine-flanked diketopyrrolopyrrole dyes”
    Spectrochim. Acta A Mol. Biomol. Spectrosc. Vol. 248, 119179 (2021). DOI: 10.1016/j.saa.2020.119179
  • Nagase K, Ishizawa Y, Inoue M, Kokubun M, Yamada S, Kanazawa H, Temperature-responsive spin column for sample preparation using an all-aqueous eluent,
    Analytica Chimica Acta, 1179, 338806, (2021).
  • Nagase K, Umemoto Y, Kanazawa H, Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns,
    Scientific Reports, 11(1), 9976, (2021).
  • Homma K, Ohta Y, Minami K, Yoshikawa G, Nagase K, Akimoto A M, Yoshida R, Autonomous Nanoscale Chemomechanical Oscillation on the Self-Oscillating Polymer Brush Surface by Precise Control of Graft Density,
    Langmuir, 37(14), 4380-4386, (2021).
  • Nomoto D, Nagase K, Nakamura Y, Kanazawa H, Citterio D, Hiruta Y, Anion species-triggered antibody separation system utilizing a thermo-responsive polymer column under optimized constant temperature,
    Colloids and Surfaces B: Biointerfaces, 205, 111890, (2021).
  • Nagase K, Shimura M, Shimane R, Hanaya K, Yamada S, Akimoto A M, Sugai T, Kanazawa H, Selective capture and non-invasive release of cells using a thermoresponsive polymer brush with affinity peptides,
    Biomaterials Science, 9(3), 663-674, (2021) (Selected Front Cover Article).
  • Maekawa Y, Ayano E, Nagase K, Kanazawa H, Effective Separation for New Therapeutic Modalities Utilizing Temperature-Responsive Chromatography,
    Analytical Sciences, 37, 651-660, (2021)

総説

  • Honami Echizen, Eita Sasaki, Kenjiro Hanaoka “Recent advances in detection, isolation, and imaging techniques for sulfane sulfur-containing biomolecules”
    Biomolecules, 11, 1553 (2021). DOI: 10.3390/biom11111553
  • Nagase K, Thermoresponsive interfaces obtained using poly(N-isopropylacrylamide)-based copolymer for bioseparation and tissue engineering applications,
    Advances in Colloid and Interface Science, 295, 102487 (2021). DOI: 10.1016/j.cis.2021.102487
  • Honami Echizen and Kenjiro Hanaoka “Recent Advances in Probe Design to Detect Reactive Sulfur Species and in the Chemical Reactions Employed for Fluorescence Switching”
    J. Clin. Biochem. Nutr. Vol. 68, 9-17 (2021). DOI: 10.3164/jcbn.20-18
  • 佐々木 栄太, 花岡 健二郎 “超硫黄分子を検出するための蛍光プローブ開発”
    生化学, 93, 604-612 (2021). DOI: 10.14952/SEIKAGAKU.2021.930604
  • 長瀬 健一、金澤 秀子“温度応答性高分子を利用した抗体医薬品分離技術の開発”,
    B&I バイオサイエンスとインダストリー, 79(1) 38-39 (2021)

2020年

原著論文

  • Rina Kunishige, Mai Mizoguchi, Asako Tsubouchi, Kenjiro Hanaoka, Yutaka Miura, Hiroshi Kurosu, Yasuteru Urano, Makoto Kuro-o, Masayuki Murata “Calciprotein particle-induced cytotoxicity via lysosomal dysfunction and altered cholesterol distribution in renal epithelial HK-2 cells”
    Scientific Reports, 10, 20125 (2020). DOI: 10.1038/s41598-020-77308-3
  • Ryo Tachibana, Mako Kamiya, Akihiko Morozumi, Yoshiyuki Miyazaki, Hiroyoshi Fujioka, Aika Nanjo, Ryosuke Kojima, Toru Komatsu, Tasuku Ueno, Kenjiro Hanaoka, Toshitad Yoshihara, Seiji Tobita, Yasuteru Urano “Design of spontaneously blinking fluorophores for live-cell super-resolution imaging based on quantum-chemical calculations”
    CHEMICAL COMMUNICATIONS, 56, 13173-13176 (2020). DOI: 10.1039/d0cc05126h
  • Naoya Nishi, Takashi Yamazawa, Tetsuo Sakka, Hiroki Hotta,Takayuki Ikeno, Kenjiro Hanaoka and Hiromi Takahashi “How Viscous is the Solid-like Structure at the Interface of Ionic Liquids? A Study Using Total Internal Reflection Fluorescence Spectroscopy with a Fluorescent Molecular Probe Sensitive to High Viscosity”
    Langmuir Vol. 36, 10397-10403 (2020). DOI: 10.1021/acs.langmuir.0c01528
  • Koji Numasawa, Kenjiro Hanaoka, Takayuki Ikeno, Honami Echizen, Tomoe Ishikawa, Masakazu Morimoto, Toru Komatsu, Tasuku Ueno, Yuji Ikegaya, Tetsuo Nagano and Yasuteru Urano “A cytosolically localized far-red to near-infrared rhodamine-based fluorescent probe for calcium ions”
    Analyst Vol. 145, 7736-7740 (2020). DOI: 10.1039/D0AN01739F
  • Toru Komatsu, Etsu Kyo, Haruki Ishii, Kyoji Tsuchikama, Aiko Yamaguchi, Tasuku Ueno, Kenjiro Hanaoka, and Yasuteru Urano “Antibody Clicking as a Strategy to Modify Antibody Functionalities on the Surface of Targeted Cells”
    J. Am. Chem. Soc. Vol. 142, 15644-15648 (2020). DOI: 10.1021/jacs.0c05331
  • Nami Kitajima, Kenji Takikawa, Hiroshi Sekiya, Kaname Satoh, Daisuke Asanuma, Hirokazu Sakamoto, Shodai Takahashi, Kenjiro Hanaoka, Yasuteru Urano, Shigeyuki Namiki, Masamitsu Iino, Kenzo Hirose “Real-time in vivo Imaging of Extracellular ATP in the Brain with a Hybrid-type Fluorescent Sensor”
    eLife, 9:e57544 (2020). DOI: 10.7554/eLife.57544
  • Koji Numasawa, Kenjiro Hanaoka, Naoko Saito, Yoshifumi Yamaguchi, Takayuki Ikeno, Honami Echizen, Masahiro Yasunaga, Toru Komatsu, Tasuku Ueno, Masayuki Miura, Tetsuo Nagano, Yasuteru Urano “A Fluorescent Probe for Rapid, High-Contrast Visualization of Folate-Receptor-Expressing Tumors in Vivo”
    Angew, Chem. Int. Ed., Vol. 59, pp6015-6020 (2020). DOI: 10.1002/anie.201914826
  • Keita Okada, Daisuke Mori, Yuma Makii, Hideki Nakamoto, Yasutaka Murahashi, Fumiko Yano, Song Ho Chang, Yuki Taniguchi, Hiroshi Kobayashi, Hiroaki Semba, Norihiko Takeda, Wen Piao, Kenjiro Hanaoka, Tetsuo Nagano, Sakae Tanaka, Taku Saito “Hypoxia-inducible factor-1 alpha maintains mouse articular cartilage through suppression of NF-κB signaling”
    Sci. Rep., 10, Article number: 5425 (2020). DOI: 10.1038/s41598-020-62463-4
  • Shodai Takahashi, Kenjiro Hanaoka, Yohei Okubo, Honami Echizen, Takayuki Ikeno, Toru Komatsu, Tasuku Ueno, Kenzo Hirose, Masamitsu Iino, Tetsuo Nagano, Yasuteru Urano “Rational Design of A Near-infrared Fluorescence Probe for Ca2+ Based on Phosphorus-substituted Rhodamines Utilizing Photoinduced Electron Transfer”
    Chem. Asian J., Vol. 15, pp524-530 (2020). DOI: 10.1002/asia.201901689
  • Shusuke Ogihara, Toru Komatsu, Yukihiro Itoh, Yuka Miyake,Takayoshi Suzuki, Kouichi Yanagi, Yusuke Kimura, Tasuku Ueno, Kenjiro Hanaoka, Hirotatsu Kojima, Takayoshi Okabe, Tetsuo Nagano, Yasuteru Urano “Metabolic Pathway-oriented Screening Targeting S-Adenosyl-L-methionine Reveals the Epigenetic Remodeling Activities of Naturally Occurring Catechols”
    J. Am. Chem. Soc., Vol. 142, pp21-26 (2020). DOI: 10.1021/jacs.9b08698
  • Nagase K, Kitazawa S, Yamada S, Akimoto A M, Kanazawa H, Mixed polymer brush as a functional ligand of silica beads for temperature-modulated hydrophobic and electrostatic interactions,
    Analytica Chimica Acta, 1095, 1-13, (2020) (Selected Front Cover Article)
  • Nagase K, Uchikawa N, Hirotani T, Akimoto A M, Kanazawa H, Thermoresponsive anionic copolymer brush-grafted surfaces for cell separation,
    Colloids and Surfaces B: Biointerfaces, 185, 110565, (2020)
  • Nagase K, Shukuwa R, Takahashi H, Takeda N, Okano T, Enhanced mechanical properties and cell separation with thermal control of PIPAAm-brushed polymer-blend microfibers,
    Journal of Materials Chemistry B, 8, 6017-6026, (2020) (Selected Front Cover Article)
  • Nishimoto T, Matsukawa K, Nagase K, Kanazawa H, Akimoto A M, Yoshida R, Design of two complementary copolymers that work as a glue for cell-laden collagen gels,
    Chemical Communications, 56, 10545-10548, (2020)
  • Maekawa Y, Okamoto N, Okada Y, Nagase K, Kanazawa H, Green analytical method for the simultaneous analysis of cytochrome P450 probe substrates by poly(N-isopropylacrylamide)-based temperature-responsive chromatography,
    Scientific Reports, 10(1), 8828, (2020)
  • Nagase K, Ota A, Hirotani T, Yamada S, Akimoto A M, Kanazawa H, Thermoresponsive Cationic Block Copolymer Brushes for Temperature-Modulated Stem Cell Separation,
    Macromolecular Rapid Communications, 41(19), 2000308, (2020) (Selected Front Cover Article)
  • Maekawa Y, Yamazaki K, Ihara M, Nagase K, Kanazawa H, Simultaneous analysis of multiple oligonucleotides by temperature-responsive chromatography using a poly(N-isopropylacrylamide)-based stationary phase,
    Analytical and Bioanalytical Chemistry, 412, 5341-5351, (2020)
  • Nagase K, Ishii S, Ikeda K, Yamada S, Ichikawa D, Akimoto A, Hattori Y, Kanazawa H, Antibody drug separation using thermoresponsive anionic polymer brush modified beads with optimised electrostatic and hydrophobic interactions.,
    Scientific Reports, 10, 11896, (2020)(新聞報道 有 3誌)
  • Nagase K, Kanazawa H, Temperature-responsive chromatography for bioseparations,
    Analytica Chimica Acta, 1138, 191-212, (2020) (Selected Front Cover Article)
  • Shao-An Wang, Chia-I Lin, Jiawei Zhang, Richiro Ushimaru, Eita Sasaki and Hung-wen Liu. “Studies of Lincosamide Formation Complete the Biosynthetic Pathway for Lincomycin A”
    Proc. Natl. Acad. Sci. U.S.A., 117, 24794–24801 (2020).
  • Eita Sasaki, Ryan M. Dragoman, Shiksha Mantri, Dmitry N. Dirin, Maksym V. Kovalenko and Donald Hilvert. “Self‐Assembly of Proteinaceous Shells Around Positively Charged Gold Nanomaterials Enhances Colloidal Stability in High Ionic Strength Buffers”
    ChemBioChem, 21, 74–79 (2020).

総説

  • 長瀬 健一、金澤 秀子“刺激応答性高分子を用いたバイオセパレーション”
    高分子, 69(9) 472-473 (2020)

著書

  • 花岡 健二郎
    “生体内pH測定のための蛍光指示薬 ―蛍光プローブの開発によるpHのバイオイメージングー”
    化学と教育,Vol.68,No.7, pp306-309 (2020).

2019年

原著論文

  • Nusaiba Madappuram Cheruthu, Toru Komatsu, Tasuku Ueno, Kenjiro Hanaoka, Yasuteru Urano “Development of Ratiometric Carbohydrate Sensor Based on Boron Dipyrromethene (BODIPY) Scaffold”
    Bioorg. Med. Chem. Lett., Vol. 29, 126684 (2019). DOI: 10.1016/j.bmcl.2019.126684
  • Yuki Ichihashi, Toru Komatsu, Etsu Kyo, Hiroyuki Matsuzaki, Keisuke Hata, Toshiaki Watanabe, Tasuku Ueno, Kenjiro Hanaoka, Yasuteru Urano “Separation-Based Enzymomics Assay for the Discovery of Altered Peptide-Metabolizing Enzymatic Activities in Biosamples”
    Anal. Chem., Vol. 91, pp11497-11501 (2019). DOI: 10.1021/acs.analchem.9b03016
  • Yuichiro Koide, Ryosuke Kojima, Kenjiro Hanaoka, Koji Numasawa, Toru Komatsu, Tetsuo Nagano, Hisataka Kobayashi, Yasuteru Urano “Design strategy for germanium-rhodamine based pH-activatable near-infrared fluorescence probes suitable for biological applications”
    Commun. Chem., 2: 94 (2019). DOI: 10.1038/s42004-019-0194-4
  • Takayuki Ikeno, Kenjiro Hanaoka,* Shimpei Iwaki, Takuya Myochin, Yoshiaki Murayama, Hisashi Ohde, Toru Komatsu, Tasuku Ueno, Tetsuo Nagano, Yasuteru Urano* “Design and Synthesis of an Activatable Photoacoustic Probe for Hypochlorous Acid”
    Anal. Chem., Vol. 91, pp9086-9092 (2019). DOI: 10.1021/acs.analchem.9b01529
  • Naoyuki Toriumi,Norihito Asano,Takayuki Ikeno,Atsuya Muranaka,Kenjiro Hanaoka,Yasuteru Urano, Masanobu Uchiyama “Design of Photostable, Activatable Near‐Infrared Photoacoustic Probes Using Tautomeric Benziphthalocyanine as a Platform”
    Angew. Chem. Int. Ed., Vol. 58, 7788-7791 (2019). DOI: 10.1002/anie.201903303
  • Akira Ogasawara, Mako Kamiya, Kei Sakamoto, Yugo Kuriki, Kyohhei Fujita, Toru Komatsu, Tasuku Ueno, Kenjiro Hanaoka, Haruna Onoyama, Hiroyuki Abe, Yosuke Tsuji, Mitsuhiro Fujishiro, Kazuhiko Koike, Masahi Fukayama Yasuyuki Seto, Yasuteru Urano “A Red Fluorescence Probe Targeted to Dipeptidylpeptidase-IV for Highly Sensitive Detection of Esophageal Cancer”
    Bioconjugate Chem., Vol. 30, pp1055-1060 (2019). DOI: 10.1021/acs.bioconjchem.9b00198
  • Tomohiro Takasugi, Kenjiro Hanaoka, Ayako Sasaki, Takayuki Ikeno, Toru Komatsu, Tasuku Ueno, Katsuya Yamada, Yasuteru Urano “Development of a Platform for Activatable Fluorescent Substrates of Glucose Transporters (GLUTs)”
    Bioorg. Med. Chem., Vol. 27, pp2122-2126 (2019). DOI: 10.1016/j.bmc.2019.02.055
  • Nagase K, Watanabe M, Zen F, Kanazawa H, Temperature-responsive mixed-mode column containing temperature-responsive polymer-modified beads and anionic polymer-modified beads,
    Analytica Chimica Acta, 1079, 220-229, (2019)
  • Nagase K, Inanaga D, Ichikawa D, Mizutani Akimoto A, Hattori Y, Kanazawa H, Temperature-modulated cell-separation column using temperature-responsive cationic copolymer hydrogel-modified silica beads,
    Colloids and Surfaces B: Biointerfaces, 178, 253-262, (2019) (Selected Front Cover Article)
  • Nagase K, Hasegawa M, Ayano E, Maitani Y, Kanazawa H, Effect of Polymer Phase Transition Behavior on Temperature-Responsive Polymer-Modified Liposomes for siRNA Transfection,
    International Journal of Molecular Sciences, 20(2), 430, (2019)
  • Maekawa-Matsuura M, Fujieda K, Maekawa Y, Nishimura T, Nagase K, Kanazawa H, LAT1-Targeting Thermoresponsive Liposomes for Effective Cellular Uptake by Cancer Cells,
    ACS Omega, 4(4), 6443-6451, (2019)
  • Nemoto R, Fujieda K, Hiruta Y, Hishida M, Ayano E, Maitani Y, Nagase K, Kanazawa H, Liposomes with temperature-responsive reversible surface properties,
    Colloids and Surfaces B: Biointerfaces, 176, 309-316, (2019)
  • Nakao M, Kim K, Nagase K, Grainger D W, Kanazawa H, Okano T, Phenotypic traits of mesenchymal stem cell sheets fabricated by temperature-responsive cell culture plate: structural characteristics of MSC sheets,
    Stem Cell Research & Therapy, 10(1), 353, (2019)
  • Nakao M, Inanaga D, Nagase K, Kanazawa H, Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins,
    Regenerative Therapy, 11, 34-40, (2019), IF: 2.286.
  • Li J, Kaku T, Tokura Y, Matsukawa K, Homma K, Nishimoto T, Hiruta Y, Akimoto A M, Nagase K, Kanazawa H, Shiratori S, Adsorption–Desorption Control of Fibronectin in Real Time at the Liquid/Polymer Interface on a Quartz Crystal Microbalance by Thermoresponsivity,
    Biomacromolecules, 20(4), 1748-1755, (2019)
  • Konishi T, Mizutani Akimoto A, Nishimoto T, Tokura Y, Tenjimbayashi M, Homma K, Matsukawa K, Kaku T, Hiruta Y, Nagase K, Kanazawa H, Shiratori S, Crosslinked Poly(N-Isopropylacrylamide)-Based Microfibers as Cell Manipulation Materials with Prompt Cell Detachment,
    Macromolecular Rapid Communications, 40(23), 1900464, (2019)
  • Homma K, Masuda T, Akimoto A M, Nagase K, Okano T, Yoshida R, Stable and Prolonged Autonomous Oscillation in a Self-Oscillating Polymer Brush Prepared on a Porous Glass Substrate,
    Langmuir, 35(30), 9794-9801, (2019)

総説

  • 越膳 ほなみ,花岡 健二郎
    “活性イオウ分子検出蛍光プローブの開発とその応用による3MST阻害剤の開発”
    日本薬理学雑誌,Vol.154, pp121-127 (2019).
  • 長瀬 健一, 金澤 秀子, 効果的な心筋細胞シート移植のためのVEGF徐放ファイバーマットの設計,
    ドラッグデリバリーシステム 34(3), 173-178, (2019).
  • 佐々木 栄太, Donald Hilvert "多価アニオン性タンパク質ケージの自己集合と構造制御"
    YAKUGAKU ZASSHI, 139, 1327–1332 (2019).

著書

  • 花岡 健二郎
    “オルガネラから臓器までのpHを可視化する”
    B&I バイオサイエンスとインダストリー (一般)バイオインダストリー協会,Vol.77,No.1 , pp10-11 (2019).
  • 花岡 健二郎
    “生きたままで生体内pHを測定できる蛍光色素の開発”
    B&I バイオサイエンスとインダストリー (一般)バイオインダストリー協会,Vol.77,No.1 , pp37-41 (2019).

2018年

原著論文

  • Hidemasa Kubo, Kenjiro Hanaoka, Yugo Kuriki, Toru Komatsu, Tasuku Ueno, Ryosuke Kojima, Mako Kamiya, Yasutoshi Murayama, Eigo Otsuji, Yasuteru Urano “Rapid Detection of Metastatic Lymph Nodes of Colorectal Cancer with A Gamma-Glutamyl Transpeptidase-Activatable Fluorescence Probe”
    Sci. Rep., Vol. 8, 17781 (2018). DOI: 10.1038/s41598-018-36062-3
  • Hiroki Ito, Yu Kawamata, Mako Kamiya, Kayoko Tsuda-Sakurai, Shinji Tanaka, Tasuku Ueno Toru Komatsu, Kenjiro Hanaoka, Shigeo Okabe, Masayuki Miura, Yasuteru Urano “Red-Shifted Fluorogenic Substrate for Detecting Lacz‐Positive Cells in Living Tissue with Single‐Cell Resolution”
    Angew. Chem. Int. Ed., Vol. 57, pp15702-15706 (2018). DOI: 10.1002/anie.201808670
  • Kenjiro Hanaoka, Yu Kagami, Wen Piao, Takuya Myochin, Koji Numasawa, Yugo Kuriki, Takayuki Ikeno, Tasuku Ueno, Toru Komatsu, Takuya Terai, Tetsuo Nagano, Yasuteru Urano “Synthesis of Unsymmetrical Si-Rhodamine Fluorophores and Application to A Far-Red to Near-Infrared Fluorescence Probe for Hypoxia”
    Chem. Commun., Vol. 54, pp6939-6942 (2018). DOI: 10.1039/c8cc02451k
  • Shodai Takahashi, Yu Kagami, Kenjiro Hanaoka, Takuya Terai, Toru Komatsu, Tasuku Ueno, Masanobu Uchiyama, Ikuko Koyama-Honda, Noboru Mizushima, Tomohiko Taguchi, Hiroyuki Arai, Tetsuo Nagano, Yasuteru Urano “Development of a Series of Practical Fluorescent Chemical Tools To Measure pH Values in Living Samples”
    J. Am. Chem. Soc., Vol. 140, pp5925-5933 (2018). DOI: 10.1021/jacs.8b00277
  • Ryo Taguchi, Takuya Terai, Tasuku Ueno , Toru Komatsu, Kenjiro Hanaoka, Yasuteru Urano “A protein-coupled fluorescent probe for organelle-specific imaging of Na+”
    Sens. Act. B: Chem., Vol. 265, pp575-581 (2018). DOI: 10.1016/j.snb.2018.03.090
  • Yugo Kuriki, Mako Kamiya, Hidemasa Kubo, Toru Komatsu, Tasuku Ueno, Ryo Tachibana, Kento Hayashi, Kenjiro Hanaoka, Suguru Yamashita, Takeaki Ishizawa, Norihiro Kokudo, Yasuteru Urano “Establishment of Molecular Design Strategy To Obtain Activatable Fluorescent Probes for Carboxypeptidases”
    J. Am. Chem. Soc., Vol. 140, 1767-1773 (2018). DOI: 10.1021/jacs.7b11014
  • Daria Ezeriņa, Yoko Takano, Kenjiro Hanaoka, Yasuteru Urano, Tobias P. Dick “N-Acetyl Cysteine Functions as a Fast-Acting Antioxidant by Triggering Intracellular H2S and Sulfane Sulfur Production”
    Cell Chem. Biol., Vol. 25, pp447-459 (2018). DOI: 10.1016/j.chembiol.2018.01.011
  • Okubo K, Ikeda K, Oaku A, Hiruta Y, Nagase K, Kanazawa H, Protein purification using solid-phase extraction on temperature-responsive hydrogel-modified silica beads,
    Journal of Chromatography A, 1568, 38-48, (2018)
  • Nagase K, Yamato M, Okano T, Temperature‐responsive Polymers for Tissue Engineering,
    Temperature‐Responsive Polymers: Chemistry, Properties and Applications, 301-312 (2018).
  • Nagase K, Yamato M, Kanazawa H, Okano T, Poly(N-isopropylacrylamide)-based thermoresponsive surfaces provide new types of biomedical applications,
    Biomaterials, 153, 27-48, (2018)
  • Nagase K, Okano T, Kanazawa H, Poly(N-isopropylacrylamide) based thermoresponsive polymer brushes for bioseparation, cellular tissue fabrication, and nano actuators,
    Nano-Structures & Nano-Objects, 16, 9-23, (2018)
  • Nagase K, Okano T, Thermoresponsive Polymer Brushes for Thermally Modulated Cell Adhesion and Detachment,
    Polymer and Biopolymer Brushes: For Materials Science and Biotechnology, pp. 361-375 (2018).
  • Matsuura M, Ohshima M, Hiruta Y, Nishimura T, Nagase K, Kanazawa H, LAT1-Targeting Thermoresponsive Fluorescent Polymer Probes for Cancer Cell Imaging,
    International Journal of Molecular Sciences, 19(6), 1646, (2018)
  • Masuda T, Akimoto A M, Furusawa M, Tamate R, Nagase K, Okano T, Yoshida R, Aspects of the Belousov–Zhabotinsky Reaction inside a Self-Oscillating Polymer Brush,
    Langmuir, 34(4), 1673-1680, (2018)
  • Akimoto A, Niitsu E, Nagase K, Okano T, Kanazawa H, Yoshida R, Mesenchylmal Stem Cell Culture on Poly(N-isopropylacrylamide) Hydrogel with Repeated Thermo-Stimulation,
    International Journal of Molecular Sciences, 19(4), 1253, (2018)
  • Kyle L. Fort, Michiel van de Waterbeemd, Dmitry Boll, Maria Reinhardt-Szyba, Mikhail E. Belov, Eita Sasaki, Reinhard Zschoche, Donald Hilvert, Alexander A. Makarov and Albert J. R. Heck, “Expanding the Structural Analysis Capabilities on an Orbitrap-Based Mass Spectrometer for Large Macromolecular Complexes”
    Analyst, 143, 100–105 (2018).

総説

  • 越膳 ほなみ, 花岡 健二郎
    “活性硫黄分子産生酵素に対する新規阻害剤の開発”
    生化学, (公益)日本生化学会, Vol.90, pp388-393 (2018).
  • 長瀬 健一, 宿輪 理紗, 小沼 隆大, 大和 雅之, 武田 直哉, 岡野 光夫, 微細構造表面と温度応答性高分子を用いた細胞分離技術の開発,
    バイオマテリアル -生体材料-, 36(2), 158-159, (2018).
  • 長瀬 健一, 関根 秀一, 清水 達也, 金澤 秀子, 岡野 光夫, Lee Seung Jin, 大和 雅之, 積層化細胞シートの移植効率向上を目的とした細胞増殖因子徐放ファイバーマットの開発,
    バイオインダストリー, 35(4), 36-45, (2018).
  • 長瀬 健一, 岡野 光夫, 金澤 秀子, 温度応答性高分子ブラシの精密設計とバイオセパレーションへの応用,
    高分子論文集, 75(2), 143-154, (2018).
  • 長瀬 健一, 金澤 秀子, 温度応答性クロマトグラフィー,
    刺激応答性高分子ハンドブック, 426-431, (2018).
  • 佐々木 栄太 “クライオ電顕による小さなタンパク質の構造解析法”
    実験医学, 36, 1356–1357 (2018).

著書

  • 花岡 健二郎,浦野 泰照
    "レドックスイメージングのための蛍光プローブ開発"
    『レドックス疾患学』実験医学(増刊号),羊土社,東京都,Vol.36,No.5,pp216-224 (2018).
  • 花岡 健二郎
    “生きた細胞や臓器のpHを測定する”
    化学 (株)化学同人, Vol.73, 11月号, pp23-28 (2018).

2017年

原著論文

  • Wen Piao, Kenjiro Hanaoka, Tomotsumi Fujisawa, Satoshi Takeuchi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Tahei Tahara, Tetsuo Nagano, Yasuteru Urano “Development of an Azo-based Photosensitizer Activated under Mild Hypoxia for Photodynamic Therapy”
    J. Am. Chem. Soc., Vol.139, pp13713-13719 (2017). DOI: 10.1021/jacs.7b05019
  • Mayumi Chiba, Yuki Ichikawa, Mako Kamiya, Toru Komatsu, Tasuku Ueno, Kenjiro Hanaoka, Tetsuo Nagano, Norbert Lange, Yasuteru Urano “An Activatable Photosensitizer Targeted to γ-Glutamyltranspeptidase”
    Angew. Chem. Int. Ed., Vol.56, pp10418-10422 (2017). DOI: 10.1002/anie.201704793
  • Naoki Muguruma, Koichi Okamoto, Tadahiko Nakagawa, Katsutaka Sannomiya, Shota Fujimoto, Yasuhiro Mitsui, Tetsuo Kimura, Hiroshi Miyamoto, Jun Higashijima, Mitsuo Shimada, Yoko Horino, Shinya Matsumoto, Kenjiro Hanaoka, Tetsuo Nagano, Makoto Shibutani, Tetsuji Takayama “Molecular imaging of aberrant crypt foci in the human colon targeting glutathione S-transferase P1-1”
    Sci. Rep., 7, 6536 (2017). DOI: 10.1038/s41598-017-06857-x
  • Tomoka Takatani-Nakase, Miku Katayama, Chihiro Matsui, Kenjiro Hanaoka, André J. van der Vlies, Koichi Takahashi, Ikuhiko Nakase, Urara Hasegawa “Hydrogen Sulfide Donor Micelles Protect Cardiomyocytes from Ischemic Cell Death”
    Mol. Biosys., Vol.13, pp1705-1708 (2017). DOI: 10.1039/C7MB00191F
  • Ryo Miyamoto, Shin Koike, Yoko Takano, Norihiro Shibuya, Yuka Kimura, Kenjiro Hanaoka, Yasuteru Urano, Yuki Ogasawara, Hideo Kimura “Polysulfides (H2Sn) Produced from the Interaction of Hydrogen Sulfide (H2S) and Nitric Oxide (NO) Activate TRPA1 Channels”
    Sci. Rep., 7, 45995 (2017). DOI: 10.1038/srep45995
  • Shingo Sakamoto, Toru Komatsu, Tasuku Ueno, Kenjiro Hanaoka, Yasuteru Urano “Fluorescence Detection of Serum Albumin with a Turnover-based Sensor Utilizing Kemp Elimination”
    Bioorg. Med. Chem. Lett., Vol.27, pp3464-3467 (2017). DOI: 10.1016/j.bmcl.2017.05.076
  • Kazuhisa Hirabayashi, Kenjiro Hanaoka, Takahiro Egawa, Chiaki Kobayashi, Shodai Takahashi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Yuji Ikegaya, Tetsuo Nagano, Yasuteru Urano “Synthesis of Practical Red Fluorescent Probe for Cytoplasmic Calcium Ions with Greatly Improved Cell-membrane Permeability”
    Data in Brief, Vol.12, pp351-357 (2017). DOI: 10.1016/j.dib.2017.04.011
  • Jun Onagi, Toru Komatsu, Yuki Ichihashi, Yugo Kuriki, Mako Kamiya, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Hiroyuki Matsuzaki, Keisuke Hata, Toshiaki Watanabe, Tetsuo Nagano, Yasuteru Urano “Discovery of Cell-type-specific and Disease-related Enzymatic Activity Changes via Global Evaluation of Peptide Metabolism”
    J. Am. Chem. Soc., Vol.139, pp3465-3472 (2017). DOI: 10.1021/jacs.6b11376
  • Narae Shin, Kenjiro Hanaoka, Wen Piao, Takuya Miyakawa, Tomotsumi Fujisawa, Satoshi Takeuchi, Shodai Takahashi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Tahei Tahara, Masaru Tanokura, Tetsuo Nagano, Yasuteru Urano “Development of an Azoreductase-based Reporter System with Synthetic Fluorogenic Substrates”
    ACS Chem. Biol., Vol.12, pp558-563 (2017). DOI: 10.1021/acschembio.6b00852
  • Yoko Takano, Kenjiro Hanaoka, Kazuhito Shimamoto, Ryo Miyamoto, Toru Komatsu, Tasuku Ueno, Takuya Terai, Hideo Kimura, Tetsuo Nagano, Yasuteru Urano “Development of a Reversible Fluorescent Probe for Reactive Sulfur Species, Sulfane Sulfur, and its Biological Application”
    Chem. Commun., Vol.53, pp1064-1067 (2017). DOI: 10.1039/C6CC08372B
  • Kenjiro Hanaoka, Kiyoshi Sasakura, Yusuke Suwanai, Sachiko Toma-Fukai, Kazuhito Shimamoto, Yoko Takano, Norihiro Shibuya, Takuya Terai, Toru Komatsu, Tasuku Ueno, Yuki Ogasawara, Yukihiro Tsuchiya, Yasuo Watanabe, Hideo Kimura, Chao Wang, Masanobu Uchiyama, Hirotatsu Kojima, Takayoshi Okabe, Yasuteru Urano, Toshiyuki Shimizu, Tetsuo Nagano “Discovery and Mechanistic Characterization of Selective Inhibitors of H2S-producing Enzyme: 3-Mercaptopyruvate Sulfurtransferase (3MST) Targeting Active-site Cysteine Persulfide"
    Sci. Rep., 7:40227 (2017). DOI: 10.1038/srep40227
  • Yusuke Kimura, Toru Komatsu, Kouichi Yanagi, Kenjiro Hanaoka, Tasuku Ueno, Takuya Terai, Hirotatsu Kojima, Takayoshi Okabe, Tetsuo Nagano, Yasuteru Urano “Development of Chemical Tools to Monitor and Control Isoaspartyl Peptide Methyltransferase Activity”
    Angew. Chem. Int. Ed., Vol.56, pp153-157 (2017). DOI: 10.1002/anie.201608677
  • Nagase K, Shukuwa R, Onuma T, Yamato M, Takeda N, Okano T, Micro/nano-imprinted substrates grafted with a thermoresponsive polymer for thermally modulated cell separation,
    Journal of Materials Chemistry B, 5(30), 5924-5930, (2017) (Selected Front Cover Article)
  • Nagase K, Sakurada Y, Onizuka S, Iwata T, Yamato M, Takeda N, Okano T, Thermoresponsive polymer-modified microfibers for cell separations,
    Acta Biomaterialia, 53, 81-92, (2017)
  • Nagase K, Nagumo Y, Kim M, Kim H-J, Kyung H-W, Chung H-J, Sekine H, Shimizu T, Kanazawa H, Okano T, Lee S-J, Yamato M, Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets,
    Macromolecular Bioscience, 17(8), 1700073, (2017) (Selected Front Cover Article).
  • Masuda T, Kajisa T, Akimoto A M, Fujita A, Nagase K, Okano T, Sakata T, Yoshida R, Dynamic electrical behaviour of a thermoresponsive polymer in well-defined poly(N-isopropylacrylamide)-grafted semiconductor devices,
    RSC Advances, 7(55), 34517-34521, (2017)
  • Homma K, Masuda T, Akimoto A M, Nagase K, Itoga K, Okano T, Yoshida R, Fabrication of Micropatterned Self-Oscillating Polymer Brush for Direction Control of Chemical Waves,
    Small, 13(21), 1700041, (2017)
  • Eita Sasaki, Daniel Böhringer, Michiel van de Waterbeemd, Marc Leibundgut, Reinhard Zschoche, Albert J. R. Heck, Nenad Ban and Donald Hilvert. “Structure and Assembly of Scalable Porous Protein Cages”
    Nat. Commun., 8, 14663 (2017).

総説

  • Yoko Takano, Honami Echizen, Kenjiro Hanaoka
    “Fluorescent Probes and Selective Inhibitors for Biological Studies of Hydrogen Sulfide- and Polysulfide-Mediated Signaling”
    Antioxid. Redox Signal., Vol.27, pp669-683 (2017).
  • Takayuki Ikeno, Tetsuo Nagano, Kenjiro Hanaoka
    “Silicon-substituted Xanthene Dyes and their Unique Photophysical Properties for Fluorescent probes”
    Chem. Asian J., Vol.12, pp1435-1446 (2017).
  • Eita Sasaki and Donald Hilvert “Self-Assembling Proteinaceous Molecular Sponges”
    Cell Syst. 4, 368 (2017).

著書

  • 花岡 健二郎
    “基礎講座 蛍光イメージングとは何か”
    現代化学 (株)東京化学同人,Vol.5, No.554, pp25-29 (2017).
  • 花岡 健二郎
    “蛍光標識試薬”
    Drug Delivery System 日本DDS学会,Vol.32, pp425-429 (2017).

2016年

原著論文

  • Toru Komatsu, Kentaro Yoshioka, Kenjiro Hanaoka, Takuya Terai Tasuku Ueno, Tetsuo Nagano, Yasuteru Urano “Identification of Lung Inflammation-Related Elevation of Acylamino Acid Releasing Enzyme (APEH) Activity Using an Enzymomics Approach”
    Chem. Pharm. Bull., Vol.11, pp1533-1538 (2016). DOI: 10.1248/cpb.c16-00540
  • Wei Chen, Armando Pacheco, Yoko Takano, Jacob J. Day, Kenjiro Hanaoka, Ming Xian “A Single Fluorescent Probe to Visualize Hydrogen Sulfide and Hydrogen Polysulfides with Different Fluorescence Signals”
    Angew. Chem. Int. Ed., Vol. 55, pp9993-9996 (2016). DOI: 10.1002/anie.201604892
  • Kazuhisa Hirabayashi, Kenjiro Hanaoka, Takahiro Egawa, Chiaki Kobayashi, Shodai Takahashi, Toru Komatsua, Tasuku Ueno, Takuya Terai, Yuji Ikegaya, Tetsuo Nagano, Yasuteru Urano “Development of Practical Red Fluorescent Probe for Cytoplasmic Calcium Ions with Greatly Improved Cell-membrane Permeability”
    Cell Calcium, Vol.60, pp256-265 (2016). DOI: 10.1016/j.ceca.2016.06.002
  • Aoi Takeda, Toru Komatsu, Hiroshi Nomura, Naka Masamitsu, Norio Matsuki, Yuji Ikegaya, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano “Unexpected photo-instability of 2,6-sulfonamide-substituted BODIPYs and its application to development of caged GABA”
    ChemBioChem, Vol.17, pp1-9 (2016). DOI: 10.1002/cbic.201600097
  • Takuya Terai, Hiroki Ito, Kenjiro Hanaoka, Toru Komatsu, Tasuku Ueno, Tetsuo Nagano, Yasuteru Urano “Detection of NAD(P)H-dependent Enzyme Activity by Time-domain Ratiometry of Terbium Luminescence”
    Bioorg. Med. Chem. Lett., Vol.6, pp2314-2317 (2016). DOI: 10.1016/j.bmcl.2016.03.038
  • Kentaro Yoshioka, Toru Komatsu, Kenjiro Hanaoka, Tasuku Ueno, Takuya Terai, Tetsuo Nagano, Yasuteru Urano “Discovery of pyruvylated peptide-metabolizing enzyme using a fluorescent substrate-based protein discovery technique”
    Chem. Commun., Vol.52, pp4377-4380 (2016). DOI: 10.1039/C6CC00829A
  • Tomoya Hirata, Takuya Terai, HisaoYamamura, Manabu Shimonishi, Toru Komatsu, Kenjiro Hanaoka, Tasuku Ueno, Yuji Imaizumi, Tetsuo Nagano, and Yasuteru Urano “A protein-coupled fluorescent probe to visualize potassium ion transition on cellular membranes”
    Anal. Chem., Vol.88, No.5, pp2693-2700 (2016). DOI: 10.1021/acs.analchem.5b03970
  • Nagase K, Okano T, Thermoresponsive-polymer-based materials for temperature-modulated bioanalysis and bioseparations,
    Journal of Materials Chemistry B, 4(39), 6381-6397, (2016)
  • Nagase K, Okano T, Bioseparation Using Thermoresponsive Polymers,
    Encyclopedia of Biocolloid and Biointerface Science, 220-230 (2016).
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermoresponsive anionic block copolymer brushes with a strongly anionic bottom segment for effective interactions with biomolecules,
    RSC Advances, 6(95), 93169-93179, (2016)
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Protein separations via thermally responsive ionic block copolymer brush layers,
    RSC Advances, 6(31), 26254-26263, (2016)
  • Nagase K, Hatakeyama Y, Shimizu T, Matsuura K, Yamato M, Takeda N, Okano T, Stem cell separation using thermoresponsive copolymer brushes having cationic charge,
    Proceedings 2015 International Symposium on MHS, 1-3, (2016).
  • Masuda T, Akimoto A M, Nagase K, Okano T, Yoshida R, Artificial cilia as autonomous nanoactuators: Design of a gradient self-oscillating polymer brush with controlled unidirectional motion,
    Science Advances, 2(8), e1600902, (2016)
  • Akimoto A M, Hasuike E, Tada H, Nagase K, Okano T, Kanazawa H, Yoshida R, Design of Tetra-arm PEG-crosslinked Thermoresponsive Hydrogel for 3D Cell Culture,
    Analytical Sciences, 32(11), 1203-1205, (2016)
  • Karsta Heinze, Eita Sasaki, Neil P. King, David Baker, Donald Hilvert, Gijs J. L. Wuite and Wouter H. Roos. “Protein Nanocontainers from Nonviral Origin: Testing the Mechanics of Artificial and Natural Protein Cages by AFM”
    J. Phys. Chem. B, 120, 5945–5952 (2016).
  • Eita Sasaki and Donald Hilvert. “Self-Assembly of Proteinaceous Multishell Structures Mediated by a Supercharged Protein”
    J. Phys. Chem. B, 120, 6089–6095 (2016).
  • Richiro Ushimaru, Chia-I Lin, Eita Sasaki and Hung-wen Liu. “Characterization of Enzymes Catalyzing Transformations of Cysteine S-Conjugated Intermediates in the Lincosamide Biosynthetic Pathway”
    ChemBioChem, 17, 1–7 (2016).
  • David R. Jackson, Xia Yu, Guojung Wang, Avinash B Patel, Jordi Calveras, Jesus F. Barajas, Eita Sasaki, Mikko Metsä-Ketelä, Hung-wen Liu, Jürgen Rohr and Shiou-Chuan Tsai. “Insights into Complex Oxidation during BE-7585A Biosynthesis: Structural Determination and Analysis of the Polyketide Monooxygenase BexE”
    ACS Chem. Biol., 11, 1137–1147 (2016).

総説

  • Yoko Takano, Kazuhito Shimamoto, Kenjiro Hanaoka
    “Chemical tools for the study of hydrogen sulfide (H2S) and sulfane sulfur and their applications to biological studies”
    J. Clin. Biochem. Nutr., Vol.58, No.1, pp7-15 (2016).
  • 長瀬健一、大和雅之 細胞シート
    バイオマテリアル-その基礎と先端研究への応用 334-337, (2016).

2015年

原著論文

  • Arnaud Chevalier, Wen Piao, Kenjiro Hanaoka, Tetsuo Nagano, Pierre-Yves Renard, Anthony Romieu “Azobenzene-caged Sulforhodamine Dyes: a Novel Class of ‘turn-on’ Reactive Probes for Hypoxic Tumor Cell Imaging”
    Methods Appl. Fluoresc., Vol.3, 044004 (2015). DOI: 10.1088/2050-6120/3/4/044004
  • Ryosuke Kojima, Hideo Takakura, Mako Kamiya, Eiji Kobayashi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano “Development of a sensitive bioluminogenic probe for imaging highly reactive oxygen species in living rats”
    Angew. Chem. Int. Ed., Vol.54, No.49, pp14768-14771 (2015). DOI: 10.1002/anie.201507530
  • Kentaro Yoshioka, Toru Komatsu, Akihiro Nakada, Jun Onagi, Yugo Kuriki, Mitsuyasu Kawaguchi, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano “Identification of tissue-restricted bio-reaction suitable for in vivo targeting by fluorescent substrate library-based enzyme discovery”
    J. Am. Chem. Soc., Vol.137, No.38, pp12187-12190 (2015). DOI: 10.1021/jacs.5b05884
  • Takuya Terai, Moe Kohno, Gaelle Boncompain, Shigeru Sugiyama, Nae Saito, Ryo Fujikake, Tasuku Ueno, Toru Komatsu, Kenjiro Hanaoka, Takayoshi Okabe, Yasuteru Urano, Franck Perez, Tetsuo Nagano “Artificial ligands of streptavidin (ALiS): discovery, characterization, and application for reversible control of intracellular protein transport”
    J. Am. Chem. Soc., Vol.137, No.33, pp10464-10467 (2015). DOI: 10.1021/jacs.5b05672
  • Kazuhisa Hirabayashi, Kenjiro Hanaoka, Toshio Takayanagi, Yuko Toki, Takahiro Egawa, Mako Kamiya, Toru Komatsu, Tasuku Ueno, Takuya Terai, Kengo Yoshida, Masanobu Uchiyama, Tetsuo Nagano, Yasuteru Urano “Analysis of chemical equilibrium of Si-substituted fluorescein and its application to develop a scaffold for red fluorescent probes”
    Anal. Chem., Vol.87, No.17, pp9061-9069 (2015). DOI: 10.1021/acs.analchem.5b02331
  • Hiroki Ito, Takuya Terai, Kenjiro Hanaoka, Tasuku Ueno, Toru Komatsu, Tetsuo Nagano, Yasuteru Urano
    “Detection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes”
    Chem. Commun., Vol.51, pp8319-8322 (2015). DOI: 10.1039/c5cc01613d
  • Takuya Myochin, Kenjiro Hanaoka, Shimpei Iwaki, Tasuku Ueno, Toru Komatsu, Takuya Terai, Tetsuo Nagano, Yasuteru Urano “Development of a series of near-infrared dark quenchers based on Si-rhodamines and their application to fluorescent probes”
    J. Am. Chem. Soc., Vol.137, No.14, pp4759-4765 (2015). DOI: 10.1021/jacs.5b00246
  • Hideo Takakura, Ryosuke Kojima, Mako Kamiya, Eiji Kobayashi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano “New class of bioluminogenic probe based on bioluminescent enzyme-induced electron transfer: BioLeT”
    J. Am. Chem. Soc., Vol.137, No.12, pp4010-4013 (2015). DOI: 10.1021/ja511014w
  • Hiroki Sakamoto, Shinichiro Egashira, Nae Saito, Takayoshi Kirisako, Simon Miller, Yoshiteru Sasaki, Tadahiko Matsumoto, Manabu Shimonishi, Toru Komatsu, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Hirotatsu Kojima, Takayoshi Okabe, Soichi Wakatsuki, Kazuhiro Iwai, Tetsuo Nagano “Gliotoxin suppresses NF-κB activation by selectively inhibiting linear ubiquitin chain assembly complex (LUBAC)”
    ACS Chem. Biol., Vol.10, No.3, pp675-681 (2015). DOI: 10.1021/cb500653y
  • Naoki Fukushima, Naoya Ieda, Mitsuyasu Kawaguchi, Kiyoshi Sasakura, Tetsuo Nagano, Kenjiro Hanaoka, Naoki Miyata, Hidehiko Nakagawa “Development of photo-controllable hydrogen sulfide donor applicable in live cells”
    Bioorg. Med. Chem. Lett., Vol.25, pp175-178 (2015). DOI: 10.1016/j.bmcl.2014.11.084
  • Nagase K, Onuma T, Yamato M, Takeda N, Okano T, Enhanced Wettability Changes by Synergistic Effect of Micro/Nanoimprinted Substrates and Grafted Thermoresponsive Polymer Brushes,
    Macromolecular Rapid Communications, 36(22), 1965-1970, (2015) (Selected Front Cover Article).
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermoresponsive hydrophobic copolymer brushes modified porous monolithic silica for high-resolution bioseparation,
    RSC Advances, 5(81), 66155-66167, (2015)
  • Nagase K, Hatakeyama Y, Shimizu T, Matsuura K, Yamato M, Takeda N, Okano T, Thermoresponsive Cationic Copolymer Brushes for Mesenchymal Stem Cell Separation,
    Biomacromolecules, 16(2), 532-540, (2015)
  • Masuda T, Terasaki A, Akimoto A M, Nagase K, Okano T, Yoshida R, Control of swelling-deswelling behavior of a self-oscillating gel by designing the chemical structure,
    RSC Advances, 5(8), 5781-5787, (2015)
  • Masuda T, Akimoto A M, Nagase K, Okano T, Yoshida R, Design of Self-Oscillating Polymer Brushes and Control of the Dynamic Behaviors,
    Chemistry of Materials, 27(21), 7395-7402, (2015)
  • Grace Caldara-Festin, David R. Jackson, Jesus F. Barajas, Timothy R. Valentic, Avinash B. Patel, Stephanie Aguilar, MyChi Nguyen, Michael Vo, Avinash Khanna, Eita Sasaki, Hung-wen Liu and Shiou-Chuan Tsai. “Structural and functional analysis of two di-domain aromatase/cyclases from type II polyketide synthases”
    Proc. Natl. Acad. Sci. U.S.A., 112, E6844–E6851 (2015).

総説

  • Kazuhito Shimamoto, Kenjiro Hanaoka
    “Fluorescent probes for hydrogen sulfide (H2S) and sulfane sulfur and their applications to biological studies”
    Nitric Oxide, Vol.46, pp72-79 (2015).
  • Yu Kushida, Tetsuo Nagano, Kenjiro Hanaoka
    “Silicon-substituted xanthene dyes and their applications in bioimaging”
    Analyst, Vol.140, pp685-695 (2015).
  • 長瀬健一 精密重合法によるナノバイオインターフェイスの構築とバイオセパレーションへの応用,
    バイオマテリアル -生体材料-, 33(1), 16-20, (2015).

2014年

原著論文

  • Toru Komatsu, Aoi Takeda, Kenjiro Hanaoka, Takuya Terai, Tasuku Ueno, Yukio Tada, Tetsuo Nagano, Yasuteru Urano “Fluorometric assay of integrin activity with a small-molecular probe that senses the binding site microenvironment”
    Chem. Commun., Vol.50, pp15894-15896 (2014). DOI: 10.1039/C4CC05591H
  • Eizo Marutani, Masahiro Sakaguchi, Wei Chen, Kiyoshi Sasakura, Jifeng Liu, Ming Xian, Kenjiro Hanaoka, Tetsuo Nagano, Fumito Ichinose “Cytoprotective effects of hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonists mediated by intracellular sulfane sulfur”
    MedChemComm., Vol.5, pp1577-1583 (2014). DOI: 10.1039/C4MD00180J
  • Nobuhiro Umeda, Hironori Takahashi, Mako Kamiya, Tasuku Ueno, Toru Komatsu, Takuya Terai, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano “Boron dipyrromethene as a fluorescent caging group for single-photon uncaging with long-wavelength visible light”
    ACS Chem. Biol., Vol.9, No.10, pp2242-2246 (2014). DOI: 10.1021/cb500525p
  • Shimpei Iwaki, Kazuya Hokamura, Mikako Ogawa, Yasuo Takehara, Yasuaki Muramatsu, Takehiro Yamane, Kazuhisa Hirabayashi, Yuji Morimoto, Kohsuke Hagisawa, Kazuhide Nakahara, Tomoko Mineno, Takuya Terai, Toru Komatsu, Tasuku Ueno, Keita Tamura, Yusuke Adachi, Yasunobu Hirata, Makoto Arita, Hiroyuki Arai, Kazuo Umemura, Tetsuo Nagano, Kenjiro Hanaoka “Design strategy for small molecule-based targeted MRI contrast agents: application for detection of atherosclerotic plaques”
    Org. Biomol. Chem., Vol.12, pp8611-8618 (2014). DOI: 10.1039/C4OB01270D
  • Hiroki Onuma, Toru Komatsu, Makoto Arita, Kenjiro Hanaoka, Tasuku Ueno, Takuya Terai, Tetsuo Nagano, Takanari Inoue “Rapidly rendering cells phagocytic through a cell-surface display technique and concurrent Rac activation”
    Sci. Signaling, Vol.7, 334, rs4 (2014). DOI: 10.1126/scisignal.2005123
  • Masataka Togashi, Takuya Terai, Hirotatsu Kojima, Kenjiro Hanaoka, Kazuei Igarashi, Yasunobu Hirata, Yasuteru Urano, Tetsuo Nagano “Practical fluorescence detection of acrolein in human plasma via a two-step tethering approach”
    Chem. Commun., Vol.50, pp14946-14948 (2014). DOI: 10.1039/C4CC02578D
  • Yoshimitsu Sagara, Toru Komatsu, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Takashi Kato, Tetsuo Nagano “Thermal or mechanical stimuli-induced photoluminescence color change of molecular assembly composed of an amphiphilic anthracene derivative in water”
    Chem. Eur. J., Vol.20, No.33, pp10397-10403 (2014). DOI: 10.1002/chem.201403025
  • Yuki Ichikawa, Mako Kamiya, Fumiaki Obata, Masayuki Miura, Takuya Terai, Toru Komatsu, Tasuku Ueno, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano “Selective ablation of β-galactosidase-expressing cells with a rationally designed activatable photosensitizer”
    Angew. Chem. Int. Ed., Vol.53, No.26, pp6772-6775 (2014). DOI: 10.1002/anie.201403221
  • Halina Jurkowska, Heather B. Roman, Lawrence L. Hirschberger, Kiyoshi Sasakura, Tetsuo Nagano, Kenjiro Hanaoka, Jakub Krijt, Martha H. Stipanuk “Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate”
    Amino Acids, Vol.46, No.5, pp1353-1365 (2014). DOI: 10.1007/s00726-014-1700-8
  • Yoshimitsu Sagara, Toru Komatsu, Tasuku Ueno, Kenjiro Hanaoka, Takashi Kato, Tetsuo Nagano “Covalent attachment of mechanoresponsive luminescent micelles to glasses and polymers in aqueous conditions”
    J. Am. Chem. Soc., Vol.136, No.11, pp4273-4280 (2014). DOI: 10.1021/ja412670g
  • Mika Mizunuma, Hiroaki Norimoto, Kentaro Tao, Takahiro Egawa, Kenjiro Hanaoka, Tetsuya Sakaguchi, Hiroyuki Hioki, Takeshi Kaneko, Shun Yamaguchi, Tetsuo Nagano, Norio Matsuki, Yuji Ikegaya “Unbalanced excitability underlies offline reactivation of behaviorally activated neurons”
    Nat. Neurosci., Vol.17, pp503-505 (2014). DOI: 10.1038/nn.3674
  • Naoki Fukushima, Naoya Ieda, Kiyoshi Sasakur, Tetsuo Nagano, Kenjiro Hanaoka, Takayoshi Suzuki, Naoki Miyata, Hidehiko Nakagawa “Synthesis of a photocontrollable hydrogen sulfide donor using ketoprofenate photocages”
    Chem. Commun., Vol.50, pp587-589 (2014). DOI: 10.1039/C3CC47421F
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermoresponsive Anionic Copolymer Brushes Containing Strong Acid Moieties for Effective Separation of Basic Biomolecules and Proteins,
    Biomacromolecules, 15(10), 3846-3858, (2014)
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Monolithic Silica Rods Grafted with Thermoresponsive Anionic Polymer Brushes for High-Speed Separation of Basic Biomolecules and Peptides,
    Biomacromolecules, 15(4), 1204-1215, (2014)
  • Nagase K, Geven M, Kimura S, Kobayashi J, Kikuchi A, Akiyama Y, Grijpma D W, Kanazawa H, Okano T, Thermoresponsive Copolymer Brushes Possessing Quaternary Amine Groups for Strong Anion-Exchange Chromatographic Matrices,
    Biomacromolecules, 15(3), 1031-1043, (2014)
  • Matsuura K, Utoh R, Nagase K, Okano T, Cell sheet approach for tissue engineering and regenerative medicine,
    Journal of Controlled Release, 190(0), 228-239, (2014)
  • Ayano E, Suzuki Y, Nishio T, Nagata Y, Kanazawa H, Nagase K, Okano T, Liquid Chromatography-Mass Spectrometric Analysis of Dehydroepiandrosterone and Related Steroids Utilizing a Temperature-Responsive Stationary Phase,
    Chromatography, 131-138, (2014).
  • Eita Sasaki, Xuan Zhang, He G. Sun, Mei-Yeh Jade Lu, Tsung-lin Liu, Albert Ou, Jeng-yi Li, Yu-hsiang Chen, Steven E. Ealick and Hung-wen Liu. “Co-opting sulphur-carrier proteins from primary metabolic pathways for 2-thiosugar biosynthesis”
    Nature, 510, 427–431 (2014).
  • Chia-I Lin, Eita Sasaki, Aoshu Zhong and Hung-wen Liu. “In Vitro Characterization of LmbK and LmbO: Identification of GDP-D-erythro-a-D-gluco-octose as a Key Intermediate in Lincomycin A Biosynthesis”
    J. Am. Chem. Soc., 136, 906–909 (2014).

総説

  • 花岡 健二郎
    “ケイ素置換キサンテン蛍光色素の開発と蛍光プローブへの展開”
    ケイ素化学協会誌, Vol.31, pp5-14 (2014).
  • 花岡 健二郎
    “赤色から近赤外蛍光団の開発による蛍光プローブ研究の展開”
    日本分子イメージング学会機関誌, Vol.8, No.1, pp28-38 (2014).
  • 長瀬健一、岡野光夫、温度応答性細胞培養表面の作製方法とその特性,
    ゲルテクノロジーハンドブック, 758-763 (2014)

著書

  • 花岡 健二郎
    "蛍光プローブが拓く新しいスクリーニング系"
    『アカデミア創薬の戦略と実例』実験医学(増刊号), 羊土社, 東京都, Vol.32, No.2, pp64-65 (2014).
  • 串田 優、花岡 健二郎、長野 哲雄
    “新規赤色蛍光色素の開発とプロテアーゼ活性検出プローブへの応用”
    ケミカルバイオロジー(日本ケミカルバイオロジー学会 編), 第7巻, 第1号, pp6-9 (2014).

2013年

原著論文

  • Wen Piao, Satoru Tsuda, Yuji Tanaka, Satoshi Maeda, Fengyi Liu, Shodai Takahashi, Yu Kushida, Toru Komatsu, Tasuku Ueno, Takuya Terai, Toru Nakazawa, Masanobu Uchiyama, Keiji Morokuma, Tetsuo Nagano, Kenjiro Hanaoka “Development of azo-based fluorescent probes to detect different levels of hypoxia”
    Angew. Chem. Int. Ed., Vol.52, No.49, pp13028-13032 (2013). DOI: 10.1002/anie.201305784
  • Toru Komatsu, Kenjiro Hanaoka, Alexander Adibekian, Takuya Terai, Tasuku Ueno, Mitsuyasu Kawaguchi, Benjamin Cravatt, Tetsuo Nagano “Diced electrophoresis gel assay for screening enzymes with specified activities”
    J. Am. Chem. Soc., Vol.135, No.16, pp6002-6005 (2013). DOI: 10.1021/ja401792d
  • Yoshimitsu Sagara, Toru Komatsu, Tasuku Ueno, Kenjiro Hanaoka, Takashi Kato, Tetsuo Nagano “A water-soluble mechanochromic luminescent pyrene derivative exhibiting recovery of the initial photoluminescence color in a high-humidity environment”
    Adv. Funct. Mater., Vol.23, No.42, pp5277-5284 (2013). DOI: 10.1002/adfm.201300180
  • Takahiro Egawa, Kazuhisa Hirabayashi, Yuichiro Koide, Chiaki Kobayashi, Naoya Takahashi, Tomoko Mineno, Takuya Terai, Tasuku Ueno, Toru Komatsu, Yuji Ikegaya, Norio Matsuki, Tetsuo Nagano, Kenjiro Hanaoka “Red fluorescence probe for monitoring dynamics of cytoplasmic calcium ion”
    Angew. Chem. Int. Ed., Vol.52, No.14, pp38740-3877 (2013). DOI: 10.1002/anie.201210279
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermally Modulated Cationic Copolymer Brush on Monolithic Silica Rods for High-Speed Separation of Acidic Biomolecules,
    ACS Applied Materials & Interfaces, 5(4), 1442-1452, (2013)
  • Nagase K, Hatakeyama Y, Shimizu T, Matsuura K, Yamato M, Takeda N, Okano T, Hydrophobized Thermoresponsive Copolymer Brushes for Cell Separation by Multistep Temperature Change,
    Biomacromolecules, 14(10), 3423-3433, (2013)
  • Masuda T, Hidaka M, Murase Y, Akimoto A M, Nagase K, Okano T, Yoshida R, Self-Oscillating Polymer Brushes,
    Angewandte Chemie International Edition, 52(29), 7468-7471, (2013)

著書

  • 花岡 健二郎、朴 文、長野 哲雄
    “アゾ基を利用した低酸素環境検出蛍光プローブの開発と応用”
    ケミカルバイオロジー (日本ケミカルバイオロジー学会 編), 第6巻, 第2号, pp2-5 (2013).
  • 高橋 翔大、花岡 健二郎、長野 哲雄
    “可逆的低酸素環境検出蛍光プローブの開発”
    日本分子イメージング学会機関誌, Vol.6, No.1, pp14-15 (2013).
  • 花岡 健二郎, 長野 哲雄
    『化学便覧 応用化学編 第7版』「16.2.4 ケミカルバイオロジー」
    丸善出版, 日本化学会編, 東京都, pp1020-1024 (2013).

2012年

原著論文

  • Shodai Takahashi, Wen Piao, Yuriko Matsumura, Toru Komatsu, Tasuku Ueno, Takuya Terai, Toshiaki Kamachi, Masahiro Kohno, Tetsuo Nagano, Kenjiro Hanaoka “Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells”
    J. Am. Chem. Soc., Vol.134, No.48, pp19588-19591 (2012). DOI: 10.1021/ja310049d
  • Takuya Myochin, Kenjiro Hanaoka, Toru Komatsu, Takuya Terai, Tetsuo Nagano "Design strategy for a near-infrared (NIR) fluorescence probe for matrix metalloproteinase utilizing highly cell-permeable boron dipyrromethene (BODIPY)"
    J. Am. Chem. Soc., vol.134, No.33, pp13730-13737 (2012). DOI: 10.1021/ja303931b
  • Takuya Terai, Rie Tomiyasu, Tomoe Ota, Tasuku Ueno, Toru Komatsu, Kenjiro Hanaoka, Yasuteru Urano, Tetsuo Nagano "TokyoGreen derivatives as specific and practical fluorescent probes for UDP-glucuronosyltransferase (UGT) 1A1"
    Chem. Comm., Vol.49, pp3101-3103 (2013). DOI: 10.1039/C3CC38810G
  • Masayo Sakabe, Daisuke Asanuma, Mako Kamiya, Ryu Iwatate, Kenjiro Hanaoka, Takuya Terai, Tetsuo Nagano, Yasuteru Urano "Rational design of highly sensitive fluorescence probes for protease and glycosidase based on precisely controlled spirocyclization"
    J. Am. Chem. Soc., vol.135, No.1, pp409-414 (2013). DOI: 10.1021/ja309688m
  • Tomonori Kobayashi, Toru Komatsu, Mako Kamiya, Cláudia Campos, Marcos González-Gaitán, Takuya Terai, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano "Highly activatable and environment-insensitive optical highlighters for selective spatiotemporal imaging of target proteins"
    J. Am. Chem. Soc., vol.134, No.27, pp11153-11160 (2012). DOI: 10.1021/ja212125w
  • Yu Kushida, Kenjiro Hanaoka, Toru Komatsu, Takuya Terai, Tasuku Ueno, Kengo Yoshida, Masanobu Uchiyama, Tetsuo Nagano "Red fluorescent scaffold for highly sensitive protease activity probes"
    Bioorg. Med. Chem. Lett., Vol.22, No.12, pp3908-3911 (2012). DOI: 10.1016/j.bmcl.2012.04.114
  • Daihi Oushiki, Hirotatsu Kojima, Yuki Takahashi, Toru Komatsu, Takuya Terai, Kenjiro Hanaoka, Makiya Nishikawa, Yoshinobu Takakura, Tetsuo Nagano "Near-infrared fluorescence probes for enzymes based on binding affinity modulation of squarylium dye scaffold"
    Anal. Chem., Vol.84, No.10, pp4404-4410 (2012). DOI: 10.1021/ac300061a
  • Yuichiro Koide, Yasuteru Urano, Kenjiro Hanaoka, Wen Piao, Moriaki Kusakabe, Nae Saito, Takuya Terai, Takayoshi Okabe, Tetsuo Nagano "Development of NIR fluorescent dyes based on Si-rhodamine for in vivo imaging"
    J. Am. Chem. Soc., Vol.134, No.11, pp5029-5031 (2012). DOI: 10.1021/ja210375e
  • Shimpei Iwaki, Kenjiro Hanaoka, Wen Piao, Toru Komatsu, Tasuku Ueno, Takuya Terai, Tetsuo Nagano "Development of hypoxia-sensitive Gd3+-based MRI contrast agents"
    Bioorg. Med. Chem. Lett., Vol.22, No.8, pp2798-2802 (2012). DOI: 10.1016/j.bmcl.2012.02.071
  • Yuichiro Koide, Mitsuyasu Kawaguchi, Yasuteru Urano, Kenjiro Hanaoka, Toru Komatsu, Takuya Terai, Tetsuo Nagano "A reversible near-infrared fluorescence probe for reactive oxygen species based on Te-rhodamine"
    Chem. Commun., Vol.48, pp3091-3093 (2012). DOI: 10.1039/C2CC18011A
  • Tamura A, Nishi M, Kobayashi J, Nagase K, Yajima H, Yamato M, Okano T, Simultaneous Enhancement of Cell Proliferation and Thermally Induced Harvest Efficiency Based on Temperature-Responsive Cationic Copolymer-Grafted Microcarriers,
    Biomacromolecules, 13(6), 1765-1773, (2012)
  • Pirraco R P, Yamato M, Akiyama Y, Nagase K, Nakayama M, Marques A P, Reis R L, Okano T, Responsive Polymer Coatings for Smart Applications in Chromatography, Drug Delivery Systems, and Cell Sheet Engineering,
    Intelligent Surfaces in Biotechnology, pp. 321-354 (2012).
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, High Stability of Thermoresponsive Polymer-Brush-Grafted Silica Beads as Chromatography Matrices,
    ACS Applied Materials & Interfaces, 4(4), 1998-2008, (2012)
  • Nagase K, Mukae N, Kikuchi A, Okano T, Thermally Modulated Retention of Lymphocytes on Polymer-Brush-Grafted Glass Beads,
    Macromolecular Bioscience, 12(3), 333-340, (2012) (Selected Front Cover Article).
  • Nagase K, Kimura A, Shimizu T, Matsuura K, Yamato M, Takeda N, Okano T, Dynamically cell separating thermo-functional biointerfaces with densely packed polymer brushes,
    Journal of Materials Chemistry, 22(37), 19514-19522, (2012)
  • Kimura A, Nagase K, Shimizu T, Matsuura K, Yamato M, Takeda N, Okano T, Development of label-free cell separating system based on the functional surface with thermoresponsive polymer brush,
    Journal of Tissue Engineering and Regenerative Medicine, 6, 206, (2012).
  • Nagase K, Mukae N, Kikuchi A, Okano T, Lymphocyte separation columns using thermoresposnive polymer brush grafted glass beads,
    Journal of Tissue Engineering and Regenerative Medicine, 6, 207, (2012).
  • Nagase K, Kimura A, Shimizu T, Matsuura K, Yamato M, Takeda N, Okano T, Dynamically cell separating thermo-responsive biointerfaces having dense polymer brushes,
    16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 6, 1105-1107, (2012).
  • Eita Sasaki, Chia-I. Lin, Ke-Yi Lin and Hung-wen Liu. “Construction of the Octose 8-Phosphate Intermediate in Lincomycin A Biosynthesis: Characterization of the Reactions Catalyzed by LmbR and LmbN”
    J. Am. Chem. Soc., 134, 17432–17435 (2012).

著書

  • 花岡 健二郎、長野 哲雄
    "3章 バイオセンシングの歴史と将来展望"
    『CSJカレントレビュー第10巻 ここまで進んだバイオセンシング・イメージング』 化学同人, 日本化学会編, 京都市, pp15-19 (2012).
  • 花岡 健二郎
    "機能性MRI造影剤の分子設計および動脈硬化イメージング"
    『疾患克服を目指したケミカルバイオロジー』実験医学(増刊号), 羊土社, 東京都, Vol.30, No.7, pp1120-1127 (2012).

2011年

原著論文

  • Kiyoshi Sasakura, Kenjiro Hanaoka, Norihiro Shibuya, Yoshinori Mikami, Yuka Kimura, Toru Komatsu, Tasuku Ueno, Takuya Terai, Hideo Kimura, Tetsuo Nagano "Development of a highly selective fluorescence probe for hydrogen sulfide"
    J. Am. Chem. Soc., Vol.133, No.45, pp18003-18005 (2011). DOI: 10.1021/ja207851s
  • Kazuhisa Hirabayashi, Kenjiro Hanaoka, Manabu Shimonishi, Takuya Terai, Toru Komatsu, Tasuku Ueno, Tetsuo Nagano “Selective two-step labeling of proteins with an off-on fluorescence probe”
    Chem. Eur. J., Vol.17, No.52, pp14763-14771 (2011). DOI: 10.1002/chem.201102664
  • Takehiro Yamane, Kenjiro Hanaoka, Yasuaki Muramatsu, Keita Tamura, Yusuke Adachi, Yasushi Miyashita, Yasunobu Hirata, Tetsuo Nagano “Method for enhancing cell-penetration of Gd3+-based MRI contrast agents by conjugation with hydrophobic fluorescent dyes”
    Bioconjugate Chem., Vol.22, No.11, pp2227-2236 (2011). DOI: 10.1021/bc200127t
  • Takahiro Egawa, Kenjiro Hanaoka, Yuichiro Koide, Sakiko Ujita, Naoya Takahashi, Yuji Ikegaya, Norio Matsuki, Takuya Terai, Tasuku Ueno, Toru Komatsu, Tetsuo Nagano "Development of a far-red to near-infrared fluorescence probe for calcium ion and its application to multicolor neuronal imaging"
    J. Am. Chem. Soc., Vol.133, No.36, pp14157-14159 (2011). DOI: 10.1021/ja205809h
  • Tomoya Hirata, Takuya Terai, Toru Komatsu, Kenjiro Hanaoka, Tetsuo Nagano "Development of a potassium ion-selective fluorescent sensor based on 3-styrylated BODIPY"
    Bioorg. Med. Chem. Lett., Vol.21, No.20, pp6090-6093 (2011). DOI: 10.1016/j.bmcl.2011.08.056
  • Toru Komatsu, Daihi Oushiki, Aoi Takeda, Masaki Miyamura, Tasuku Ueno , Takuya Terai, Kenjiro Hanaoka, Yasuteru Urano, Tomoko Mineno, Tetsuo Nagano "Rational design of boron dipyrromethene (BODIPY)-based photobleaching-resistant fluorophore applicable to protein dynamics study"
    Chem. Commun., Vol.47, pp10055-10057 (2011). DOI: 10.1039/C1CC13367E
  • Mitsuyasu Kawaguchi, Takayoshi Okabe, Shinichi, Okudaira, Kenjiro Hanaoka, Yuuta Fujikawa, Takuya Terai, Toru Komatsu, Hirotatsu Kojima, Junken Aoki, Tetsuo Nagano "Fluorescence probe for lysophospholipase C/NPP6 activity and a potent NPP6 inhibitor"
    J. Am. Chem. Soc., Vol.133, No.31, pp12021-12030 (2011). DOI: 10.1021/ja201028t
  • Masahiro Abo, Yasuteru Urano, Kenjiro Hanaoka, Takuya Terai, Toru Komatsu, Tetsuo Nagano "Development of a highly sensitive fluorescence probe for hydrogen peroxide"
    J. Am. Chem. Soc., Vol.133, No.27, pp10629-10637 (2011). DOI: 10.1021/ja203521e
  • Yuichiro Koide, Yasuteru Urano, Kenjiro Hanaoka, Takuya Terai, Tetsuo Nagano "Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and applications for biological imaging"
    J. Am. Chem. Soc., Vol.133, No.15, pp5680-5682 (2011). DOI: 10.1021/ja111470n
  • Mitsuyasu Kawaguchi, Kenjiro Hanaoka, Toru Komatsu, Takuya Terai, Tetsuo Nagano "Development of a highly selective fluorescence probe for alkaline phosphatase"
    Bioorg. Med. Chem. Lett., Vol.21, No.17, pp5088-5091 (2011). DOI: 10.1016/j.bmcl.2011.03.070
  • Yuichiro Koide, Yasuteru Urano, Kenjiro Hanaoka, Takuya Terai, Tetsuo Nagano "Evolution of group 14 rhodamines as platforms for near-infrared fluorescence probes utilizing photoinduced electron transfer"
    ACS Chem. Biol., Vol.6, No.6, pp600-608 (2011). DOI: 10.1021/cb1002416
  • Takahiro Egawa, Yuichiro Koide, Kenjiro Hanaoka, Toru Komatsu, Takuya Terai, Tetsuo Nagano "Development of fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region"
    Chem. Commun., Vol.47, pp4162-4164 (2011). DOI: 10.1039/C1CC00078K
  • Takuya Myochin, Kazuki Kiyose, Kenjiro Hanaoka, Hirotatsu Kojima, Takuya Terai, Tetsuo Nagano "Rational design of ratiometric near-infrared fluorescent pH probes with various pKa values, based on aminocyanine"
    J. Am. Chem. Soc., Vol.133, No.10, pp3401-3409 (2011). DOI: 10.1021/ja1063058
  • Hideo Takakura, Ryosuke Kojima, Yasuteru Urano, Takuya Terai, Kenjiro Hanaoka, Tetsuo Nagano "Aminoluciferins as functional bioluminogenic substrates of firefly luciferase"
    Chem. Asian J., Vol.6, No.7, pp1800-1810 (2011). DOI: 10.1002/asia.201000873
  • Naomi Takiguchi, Yuki Takahashi, Makiya Nishikawa, Yuriko Matsui, Yasushi Fukuhara, Daihi Oushiki, Kazuki Kiyose, Kenjiro Hanaoka, Tetsuo Nagano, Yoshinobu Takakura "Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice"
    Pharm. Res., Vol.28, No.4, pp702-711 (2011). DOI: 10.1007/s11095-010-0331-3
  • Nagase K, Yuk S F, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermo-responsive protein adsorbing materials for purifying pharmaceutical protein on exposed charging surface,
    Journal of Materials Chemistry, 21(8), 2590-2593, (2011)
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermally-modulated on/off-adsorption materials for pharmaceutical protein purification,
    Biomaterials, 32(2), 619-627, (2011)
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermoresponsive Polymer Brush on Monolithic-Silica-Rod for the High-Speed Separation of Bioactive Compounds,
    Langmuir, 27(17), 10830-10839, (2011)
  • Nagase K, Watanabe M, Kikuchi A, Yamato M, Okano T, Thermo-Responsive Polymer Brushes as Intelligent Biointerfaces: Preparation via ATRP and Characterization,
    Macromolecular Bioscience, 11(3), 400-409, (2011) (Selected Front Cover Article).
  • Nagase K, Mizutani Akimoto A, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Effect of reaction solvent on the preparation of thermo-responsive stationary phase through a surface initiated atom transfer radical polymerization,
    Journal of Chromatography A, 1218(48), 8617-8628, (2011)

著書

  • 花岡 健二郎, 長野 哲雄
    "MRI造影剤"
    『蛍光イメージング/MRIプローブの開発と最新動向』, シーエムシー出版, 東京都, pp59-69 (2011).
  • 篠倉 潔、花岡 健二郎、木村 英雄、長野 哲雄
    “硫化水素選択的蛍光プローブの開発”
    ケミカルバイオロジー (日本ケミカルバイオロジー学会 編), 第4巻, 第2号, pp6-9 (2011).
  • 花岡 健二郎、村松 泰明、山根 健浩、平田 恭信、長野 哲雄
    “動脈硬化巣の可視化を目指したMRIプローブの開発”
    日本分子イメージング学会機関誌, Vol.4, No.1, pp16-18 (2011).

2010年

原著論文

  • Kazuki Kiyose, Kenjiro Hanaoka, Daihi Oushiki, Tomomi Nakamura, Mayumi Kajimura, Makoto Suematsu, Hiroaki Nishimatsu, Takehiro Yamane, Takuya Terai, Tasunobu Hirata, Tatsuo Nagano "Hypoxia-sensitive fluorescent probes for in vivo real-time fluorescence imaging of acute ischemia"
    J. Am. Chem. Soc., Vol.132, No.45, pp15846-15848 (2010). DOI: 10.1021/ja105937q
  • Mitsuyasu Kawaguchi, Takayoshi Okabe, Takuya Terai, Kenjiro Hanaoka, Hirotatsu Kojima, Izumi Minegishi, Tetsuo Nagano "A time-resolved fluorescence probe for dipeptidyl peptidase 4 and its application for inhibitor screening"
    Chem. Eur. J., Vol.16, No.45, pp13479-13486 (2010). DOI: 10.1002/chem.201001077
  • Hiromi Sasaki, Kenjiro Hanaoka, Yasuteru Urano, Takuya Terai, Tetsuo Nagano "Design and synthesis of a novel fluorescence probe for Zn2+ based on the spirolactam ring−opening process of rhodamine derivatives"
    Bioorg. Med. Chem., Vol.19, No.3, pp1072-1078 (2011). DOI: 10.1016/j.bmc.2010.05.074
  • Hideo Takakura, Kiyoshi Sasakura, Tasuku Ueno, Yasuteru Urano, Takuya Terai, Kenjiro Hanaoka, Takashi Tsuboi, Tetsuo Nagano "Development of luciferin analogues bearing an amino group and their application as BRET donors"
    Chem. Asian J., Vol.5, No.9, pp2053-2061 (2010). DOI: 10.1002/asia.201000219
  • Masataka Togashi, Yasuteru Urano, Hirotatsu Kojima, Takuya Terai, Kejiro Hanaoka, Kazuei Igarashi, Yasunobu Hirata, Tetsuo Nagano "Sensitive detection of acrolein in serum using time-resolved luminescence"
    Org. Lett., Vol.12, No.8, pp1704-1707 (2010). DOI: 10.1021/ol1002219
  • Daihi Oushiki, Hirotatsu Kojima, Takuya Terai, Makoto Arita, Kenjiro Hanaoka, Yasuteru Urano, Tetsuo Nagano "Development and application of a near-infrared fluorescence probe for oxidative stress based on differential reactivity of linked cyanine dyes"
    J. Am. Chem. Soc., Vol.132, No.8, pp2795-2801 (2010). DOI: 10.1021/ja910090v
  • Kenjiro Hanaoka, Yasuaki Muramatsu, Yasuteru Urano, Takuya Terai, Tetsuo Nagano "Design and synthesis of a highly sensitive off-on fluorescent chemosensor for zinc ion utilizing internal charge transfer"
    Chem. Eur. J., Vol.16, No.2, pp568-572 (2010). DOI: 10.1002/chem.200901591
  • Nagase K, Kumazaki M, Kanazawa H, Kobayashi J, Kikuchi A, Akiyama Y, Annaka M, Okano T, Thermoresponsive Polymer Brush Surfaces with Hydrophobic Groups for All-Aqueous Chromatography,
    ACS Applied Materials & Interfaces, 2(4), 1247-1253, (2010)
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Annaka M, Okano T, Preparation of Thermoresponsive Anionic Copolymer Brush Surfaces for Separating Basic Biomolecules,
    Biomacromolecules, 11(1), 215-223, (2010)
  • Mizutani A, Nagase K, Kikuchi A, Kanazawa H, Akiyama Y, Kobayashi J, Annaka M, Okano T, Effective separation of peptides using highly dense thermo-responsive polymer brush-grafted porous polystyrene beads,
    Journal of Chromatography B, 878(24), 2191-2198, (2010)
  • Mizutani A, Nagase K, Kikuchi A, Kanazawa H, Akiyama Y, Kobayashi J, Annaka M, Okano T, Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides,
    Journal of Chromatography A, 1217(38), 5978-5985, (2010)
  • Mizutani A, Nagase K, Kikuchi A, Kanazawa H, Akiyama Y, Kobayashi J, Annaka M, Okano T, Thermo-responsive polymer brush-grafted porous polystyrene beads for all-aqueous chromatography,
    Journal of Chromatography A, 1217(4), 522-529, (2010)
  • Idota N, Nagase K, Tanaka K, Okano T, Annaka M, Stereoregulation of Thermoresponsive Polymer Brushes by Surface-Initiated Living Radical Polymerization and the Effect of Tacticity on Surface Wettability,
    Langmuir, 26(23), 17781-17784, (2010)
  • Hattori Y, Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Hydration of poly(N-isopropylacrylamide) brushes on micro-silica beads measured by a fluorescent probe,
    Chemical Physics Letters, 491(4-6), 193-198, (2010)
  • Eita Sasaki and Hung-wen Liu. “Mechanistic Studies of the Biosynthesis of 2-Thiosugar: Evidence for the Formation of an Enzyme-Bound 2-Ketohexose Intermediate in BexX-Catalyzed Reaction”
    J. Am. Chem. Soc., 132, 15544–15546 (2010).
  • Eita Sasaki, Yasushi Ogasawara, and Hung-wen Liu. “A Biosynthetic Pathway for BE-7585A, a 2-Thiosugar-Containing Angucycline-Type Natural Product”
    J. Am. Chem. Soc., 132, 7405–7417 (2010).

総説

  • Kenjiro Hanaoka
    "Development of responsive lanthanide-based magnetic resonance imaging and luminescent probes for biological applications"
    Chem. Pharm. Bull., Vol.58, No.10, pp1283-1294 (2010).

2009年

原著論文

  • Toru Komatsu, Yasuteru Urano, Yuuta Fujikawa, Tomonori Kobayashi, Hirotatsu Kojima, Takuya Terai, Kenjiro Hanaoka, Tetsuo Nagano "Development of 2,6-carboxy-substituted boron dipyrromethene (BODIPY) as a novel scaffold of ratiometric fluorescent probes for live cell imaging"
    Chem. Commun., pp7015-7017 (2009). DOI: 10.1039/B917209B
  • Saki Izumi, Yasuteru Urano, Kenjiro Hanaoka, Takuya Terai, Tetsuo Nagano “A simple and effective strategy to increase the sensitivity of fluorescence probes in living cells”
    J. Am. Chem. Soc., Vol.131, No.29, pp10189-10200 (2009). DOI: 10.1021/ja902511p
  • Kazuki Kiyose, Sakiko Aizawa, Eita Sasaki, Hirotatsu Kojima, Yasuteru Urano, Kenjiro Hanaoka, Takuya Terai, Tetsuo Nagano “Molecular design strategies for near-infrared ratiometric fluorescent probes based on unique spectral properties of aminocyanines”
    Chem. Eur. J., Vol.15, No.36, pp9191-9200 (2009). DOI: 10.1002/chem.200900035
  • Yuichiro Koide, Yasuteru Urano, Akira Yatsushige, Kenjiro Hanaoka, Takuya Terai, Tetsuo Nagano “Design and development of enzymatically activatable photosensitizer based on unique characteristics of thiazole orange”
    J. Am. Chem. Soc., Vol.131, No.17, pp6058-6059 (2009). DOI: 10.1021/ja900443b
  • Nagase K, Kobayashi J, Okano T, Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering,
    Journal of The Royal Society Interface, 6(Suppl 3), S293-S309, (2009)
  • Kazuki Kiyose, Sakiko Aizawa, Eita Sasaki, Hirotatsu Kojima, Kenjiro Hanaoka, Takuya Terai, Yasuteru Urano and Tetsuo Nagano. “Molecular Design Strategies for Near-Infrared Ratiometric Fluorescent Probes Based on the Unique Spectral Properties of Aminocyanines”,
    Chem.-Eur. J., 15, 9191–9200 (2009).

著書

  • 花岡 健二郎, 長野 哲雄
    "MRI用センサー分子の分子設計・化学合成"
    化学フロンティア:新しい地平をひらく分析手法の最前線, 化学同人, 京都市, pp69-76 (2009).

2008年以前

原著論文

  • Yuuta Fujikawa, Yasuteru Urano, Toru Komatsu, Kenjiro Hanaoka, Hirotatsu Kojima, Takuya Terai, Hideshi Inoue, Tetsuo Nagano “Design and synthesis of highly sensitive fluorogenic substrates for glutathione S-transferase (GST) and application for activity imaging in living cells”
    J. Am. Chem. Soc., Vol.130, No.44, pp14533-14543 (2008). DOI: 10.1021/ja802423n
  • Kenjiro Hanaoka, Kazuya Kikuchi, Takuya Terai, Toru Komatsu, Tetsuo Nagano “A Gd3+-based magnetic resonance imaging contrast agent sensitive to b-galactosidase activity utilizing a receptor-induced magnetization enhancement (RIME) phenomenon”
    Chem. Eur. J., Vol.14, No.3, pp987-995 (2008). DOI: 10.1002/chem.200700785
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Effects of Graft Densities and Chain Lengths on Separation of Bioactive Compounds by Nanolayered Thermoresponsive Polymer Brush Surfaces,
    Langmuir, 24(2), 511-517, (2008)
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Preparation of Thermoresponsive Cationic Copolymer Brush Surfaces and Application of the Surface to Separation of Biomolecules,
    Biomacromolecules, 9(4), 1340-1347, (2008)
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Annaka M, Kanazawa H, Okano T, Influence of Graft Interface Polarity on Hydration/Dehydration of Grafted Thermoresponsive Polymer Brushes and Steroid Separation Using All-Aqueous Chromatography,
    Langmuir, 24(19), 10981-10987, (2008)
  • Kenjiro Hanaoka, Angelo Josue M. Lubag, Aminta Castillo-Muzquiz, Thomas Kodadek, A. Dean Sherry “The detection limit of a Gd3+-based T1 agent is substantially reduced when targeted to a protein microdomain”
    Magn. Reson. Imaging, Vol.26, No.5, pp608-617 (2008). DOI: 10.1016/j.mri.2007.11.002
  • Kenjiro Hanaoka, Kazuya Kikuchi, Shigeru Kobayashi, Tetsuo Nagano “Time-resolved long-lived luminescence imaging method employing luminescent lanthanide probes with a new microscopy system”
    J. Am. Chem. Soc., Vol.129, No.44, pp13502-13509 (2007). DOI: 10.1021/ja073392j
  • Michiaki Kumagai, Yutaka Imai, Teisaku Nakamura, Yuichi Yamasaki, Masaki Sekino, Shoogo Ueno, Kenjiro Hanaoka, Kazuya Kikuchi, Tetsuo Nagano, Eiji Kaneko, Kentaro Shimokado, Kazunori Kataoka “Iron hydroxide nanoparticles coated with poly(ethylene glycol)-poly(aspartic acid) block copolymer as novel magnetic resonance contrast agents for in vivo cancer imaging”
    Colloids Surf. B: Biointerfaces, Vol.56, No.1-2, pp174-181 (2007). DOI: 10.1016/j.colsurfb.2006.12.019
  • Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Interfacial Property Modulation of Thermoresponsive Polymer Brush Surfaces and Their Interaction with Biomolecules,
    Langmuir, 23(18), 9409-9415, (2007).
  • Annaka M, Yahiro C, Nagase K, Kikuchi A, Okano T, Real-time observation of coil-to-globule transition in thermosensitive poly(N-isopropylacrylamide) brushes by quartz crystal microbalance,
    Polymer, 48(19), 5713-5720, (2007)
  • Toru Komatsu, Kazuya Kikuchi, Hideo Takakusa, Kenjiro Hanaoka, Tasuku Ueno, Mako Kamiya, Yasuteru Urano, Tetsuo Nagano “Design and synthesis of an enzyme activity-based labeling molecule with fluorescence spectral change”
    J. Am. Chem. Soc., Vol.128, No.50, pp15946-15947 (2006). DOI: 10.1021/ja0657307
  • Kenjiro Hanaoka, Kazuya Kikuchi, Hirotatsu Kojima, Yasuteru Urano, Tetsuo Nagano “Development of a zinc ion-selective luminescent lanthanide chemosensor for biological applications”
    J. Am. Chem. Soc., Vol.126, No.39, pp12470-12476 (2004). DOI: 10.1021/ja0469333
  • Nagase K, Kohori F, Sakai K, Nishide H, Rearrangement of hollow fibers for enhancing oxygen transfer in an artificial gill using oxygen carrier solution,
    Journal of Membrane Science, 254(1-2), 207-217, (2005),
  • Nagase K, Kohori F, Sakai K, Oxygen transfer performance of a membrane oxygenator composed of crossed and parallel hollow fibers,
    Biochemical Engineering Journal, 24(2), 105-113, (2005)
  • Nagase K, Hasegawa U, Kohori F, Sakai K, Nishide H, The photoresponse of a molybdenum porphyrin makes an artificial gill feasible,
    Journal of Membrane Science, 249(1-2), 235-243, (2005)
  • Eita Sasaki, Hirotatsu Kojima, Hiroaki Nishimatsu, Yasuteru Urano, Kazuya Kikuchi, Yasunobu Hirata and Tetsuo Nagano. “Highly Sensitive Near-Infrared Fluorescence Probes for Nitric Oxide and Their Application to Isolated Organs”
    J. Am. Chem. Soc., 127, 3684–3685 (2005).
  • Nagase K, Hasegawa U, Kohori F, Sakai K, Nishide H, A Photoresponsive Artificial Gill,
    Proceedings of Asian Pacific Confederation of Chemical Engineering Congress, 489-494, (2004)
  • Nagase K, Kohori F, Sakai K, Efficiency of biological and artificial gills,,
    Bio-mechanisms of Swimming and Flying, 188-199, (2004)
  • Nagase K, Kohori F, Sakai K, A Compact Artificial Gill using a Hemoglobin Solution as a Thermo-Responsive Oxygen Carrier,
    Proceedings of Asian Pacific Confederation of Chemical Engineering Congress, 407-494, (2004)
  • Kenjiro Hanaoka, Kazuya Kikuchi, Hirotatsu Kojima, Yasuteru Urano, Tetsuo Nagano “Selective detection of zinc ions with novel luminescent lanthanide probes”
    Angew. Chem. Int. Ed., Vol.42, No.26, pp2996-2999 (2003). DOI: 10.1002/anie.200351122
  • Nagase K, Kohori F, Sakai K, Designing an Artificial Gill Using Oxygen Carrier Solution for Effective Supply of Oxygen to Underwater Closed Spaces,
    Journal of Research in Science and Engineering, 1, 31-36, (2003).
  • Nagase K, Kohori F, Sakai K, Development of a compact artificial gill using concentrated hemoglobin solution as the oxygen carrier,
    Journal of Membrane Science, 215(1-2), 281-292, (2003)
  • Kenjiro Hanaoka, Kazuya Kikuchi, Yasuteru Urano, Michiko Narazaki, Takashi Yokawa, Shigeru Sakamoto, Kentaro Yamaguchi, Tetsuo Nagano “Design and synthesis of a novel magnetic resonance imaging contrast agent for selective sensing of zinc ion”
    Chem. Biol., Vol.9, No.9, pp1027-1032 (2002). DOI: 10.1016/s1074-5521(02)00216-8
  • Nagase K, Matsuda N, Sakai K, Optimum control of oxygen affinity of hemoglobin as an oxygen carrier solution for an artificial gill,
    Journal of Artificial Organs, 5(1), 44-49, (2002).
  • Kenjiro Hanaoka, Kazuya Kikuchi, Yasuteru Urano, Tetsuo Nagano “Selective sensing of zinc ions with a novel magnetic resonance imaging contrast agent”
    J. Chem. Soc. –Perkin Trans. 2, pp1840-1843 (2001). DOI: 10.1039/B100994J

総説

  • 長瀬健一, 岡野光夫, 温度応答性インテリジェント表面とバイオセパレーション,
    表面技術, 59(6), 387-391, (2008).
  • 長瀬 健一, 小堀 深, 酒井 清孝, 人工鰓の技術的展望,
    日本バイオレオロジー学会誌, 15(3), 1-7, (2001).
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